Journal: Molecular Pharmaceutics

Article Title: Preparing a Dual-Species In Vitro Biofilm Model for Testing Antibiofilm Efficacy

doi: 10.1021/acs.molpharmaceut.5c00798

Figure Lengend Snippet: (A-B) E. coli and S. aureus dual-species biofilm growth using different preconditioning methods for E. coli ( N = 3). (C). Comparison of sonication and homogenization as disruption methods on counted bacteria in S. aureus and P. aeruginosa 48-h grown biofilms ( N = 3). (D) Images of S. aureus colonies on mannitol-salt agar, where the growth of Gram-negative bacteria is inhibited. (E) Images of P. aeruginosa and E. coli colonies on Tergitol-7 agar without TTC supplement, where blue arrows point to P. aeruginosa colonies and yellow arrows to E. coli colonies. Key: SA – S. aureus ; PA – P. aeruginosa ; SA mix and PA mix – S. aureus and P. aeruginosa , respectively, in a dual-species biofilm. S. aureus was prepared from the overnight liquid culture at an inoculum concentration of 10 6 CFU/mL in all the replicates. Data are presented as the mean and SD. D-E. Scale bar: 1 cm.

Article Snippet: Disruption by homogenization (Bertin Technologies Precellys Evolution Touch, 2 mL tubes, 1.4 mm CK beads) was performed at 8000 rpm for 20 s. The number of biofilm bacteria was determined by CFU plating after biofilm disruption.

Techniques: Comparison, Sonication, Homogenization, Disruption, Bacteria, Concentration Assay